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Other Glutathione-Sparing Compounds Several nutrients help maintain glutathione levels by reducing its consumption or supporting its recycling: Vitamin C (ascorbic acid): Spares glutathione by scavenging reactive oxygen species directly, reducing the demand for glutathione as an antioxidant

1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

However, the following cases should be noted: Asthma: Avoid inhaling Glutathione to prevent worsening asthma symptoms